How could I build a tool to help with this scenario :
I work in a lab where we use plasmids to express recombinant proteins. We have a database containing all the plasmid identifiers and the sequence of the protein that they code for.
When a new protein is requested, I would like to be able to input the new desired protein sequence and search in our database for the plasmid that has the closest match to that sequence, with the highest identity score. The objective is to then use that existing plasmid and use it as a cloning template for the new plasmid.
In other words, I want to build a tool similar to NCBI blast that would work locally with proprietary sequences that are in an SQL database.
Would Python be able to achieve that ?
Thanks !
How about creating your own local BLAST database with makeblastdb? Then you can use something like this:
from Bio.Blast.Applications import NcbiblastnCommandline
run_command = NcbiblastnCommandline(query=YOUR_SEQUENCE_FASTA_PATH,
db=DATABASE_PATH,
out=RESULT_PATH,
outfmt=5,
[… other parameters …],
evalue=1e-10
)
stdout, stderr = run_command()
Related
I have built an Microsoft Azure ML Studio workspace predictive web service, and have a scernario where I need to be able to run the service with different training datasets.
I know I can setup multiple web services via Azure ML, each with a different training set attached, but I am trying to find a way to do it all within the same workspace and passing a Web Input Parameter as the input value to choose which training set to use.
I have found this article, which describes almost my scenario. However, this article relies on the training dataset that is being pulled from the Load Trained Data module, as having a static endpoint (or blob storage location). I don't see any way to dynamically (or conditionally) change this location based on a Web Input Parameter.
Basically, does Azure ML support a "conditional training data" loading?
Or, might there be a way to combine training datasets, then filter based on the passed Web Input Parameter?
This probably isn't exactly what you need, but hopefully, it helps you out.
To combine data sets, you can use the Join Data module.
To filter, that may be accomplished by executing a Python script. Here's an example.
Using the Adult Census Income Binary Classification dataset, on the age column, there's a minimum age of 17.
If I wanted to filter the data set by age, connect it to an Execute Python Script module and here's the filtering code with the pandas query method.
# The script MUST contain a function named azureml_main
# which is the entry point for this module.
import pandas as pd
def azureml_main(dataframe1 = None, dataframe2 = None):
# Return value must be of a sequence of pandas.DataFrame
return dataframe1.query("age >= 25")
And looking at that output it filters out the data set where the minimum age is now 25.
Sure, you can do that. What you would want is to use an Execute R Script or SQL Transformation module to determine, based on your input data, what model to use. Something like this:
Notice, your input data is cleaned/updated/feature engineered, then it's passed to two different SQL transforms which will tell it to go to one of two paths.
Each path has it's own training data.
Note: I am not exactly sure what your use case is, but if it were me, I would instead train two different models using the two different training data, then try to just use the models in my web service, not actually train on the web service as that would likely be quite slow.
Evaluating Dataflow, and am trying to figure out if/how to do the following.
My apologies if anything in the above is trivial--trying to wrap our heads around Dataflow before we make a decision on using Beam, or something else like Spark, etc.
General use case is for machine learning:
Ingesting documents which are individually processed.
In addition to easy-to-write transforms, we'd like to enrich each document based on queries against databases (that are largely key-value stores).
A simple example would be a gazetteer: decompose the text into ngrams, and then check if those ngrams reside in some database, and record (within a transformed version of the original doc) the entity identifier given phrases map to.
How to do this efficiently?
NAIVE (although possibly tricky with the serialization requirement?):
Each document could simply query the database individually (similar to Querying a relational database through Google DataFlow Transformer), but, given that most of these are simple key-value stores, it seems like there should be a more efficient way to do this (given the real problems with database query latency).
SCENARIO #1: Improved?:
Current strawman is to store the tables in Bigquery, pull them down (https://github.com/apache/beam/blob/master/sdks/python/apache_beam/io/gcp/bigquery.py), and then use them as side inputs, that are used as key-value lookups within the per-doc function(s).
Key-value tables range from generally very small to not-huge (100s of MBs, maybe low GBs). Multiple CoGroupByKey with same key apache beam ("Side inputs can be arbitrarily large - there is no limit; we have seen pipelines successfully run using side inputs of 1+TB in size") suggests this is reasonable, at least from a size POV.
1) Does this make sense? Is this the "correct" design pattern for this scenario?
2) If this is a good design pattern...how do I actually implement this?
https://github.com/apache/beam/blob/master/sdks/python/apache_beam/io/gcp/bigquery.py#L53 shows feeding the result to the document function as an AsList.
i) Presumably, AsDict is more appropriate here, for the above use case? So I'd probably need to run some transformations first on the Bigquery output to separate it into key, value tuple; and make sure that the keys are unique; and then use it as a side input.
ii) Then I need to use the side input in the function.
What I'm not clear on:
for both of these, how to manipulate the output coming off of the Bigquery pull is murky to me. How would I accomplish (i) (assuming it is necessary)? Meaning, what does the data format look like (raw bytes? strings? is there a good example I can look into?)
Similarly, if AsDict is the correct way to pass it into the func, can I just reference things like a dict normally is used in python? e.g., side_input.get('blah') ?
SCENARIO #2: Even more improved? (for specific cases):
The above scenario--if achievable--definitely does seem like it is superior continuous remote calls (given the simple key-value lookup), and would be very helpful for some of our scenarios. But if I take a scenario like a gazetteer lookup (like above)...is there an even more optimized solution?
Something like, for every doc, writing our all the ngrams as keys, with values as the underlying indices (docid+indices within the doc), and then doing some sort of join between these ngrams and the phrases in our gazeteer...and then doing another set of transforms to recover the original docs (now w/ their new annotations).
I.e., let Beam handle all of the joins/lookups directly?
Theoretical advantage is that Beam may be a lot quicker in doing this than, for each doc, looping over all of the ngrams and doing a check if the ngram is in the side_input.
Other key issues:
3) If this is a good way to do things, is there any trick to making this work well in the streaming scenario? Text elsewhere suggests that the side input caching works more poorly outside the batch scenario. Right now, we're focused on batch, but streaming will become relevant in serving live predictions.
4) Any Beam-related reason to prefer Java>Python for any of the above? We've got a good amount of existing Python code to move to Dataflow, so would heavily prefer Python...but not sure if there are any hidden issues with Python in the above (e.g., I've noticed Python doesn't support certain features or I/O).
EDIT: Strawman? for the example ngram lookup scenario (should generalize strongly to general K:V lookup)
Phrases = get from bigquery
Docs (indexed by docid) (direct input from text or protobufs, e.g.)
Transform: phrases -> (phrase, entity) tuples
Transform: docs -> ngrams (phrase, docid, coordinates [in document])
CoGroupByKey key=phrase: (phrase, entity, docid, coords)
CoGroupByKey key=docid, group((phrase, entity, docid, coords), Docs)
Then we can iteratively finalize each doc, using the set of (phrase, entity, docid, coords) and each Doc
Regarding the scenarios for your pipeline:
Naive scenario
You are right that per-element querying of a database is undesirable.
If your key-value store is able to support low-latency lookups by reusing an open connection, you can define a global connection that is initialized once per worker instead of once per bundle. This should be acceptable your k-v store supports efficient lookups over existing connections.
Improved scenario
If that's not feasible, then BQ is a great way to keep and pull in your data.
You can definitely use AsDict side inputs, and simply go side_input[my_key] or side_input.get(my_key).
Your pipeline could look something like so:
kv_query = "SELECT key, value FROM my:table.name"
p = beam.Pipeline()
documents_pcoll = p | ReadDocuments()
additional_data_pcoll = (p
| beam.io.BigQuerySource(query=kv_query)
# Make row a key-value tuple.
| 'format bq' >> beam.Map(lambda row: (row['key'], row['value'])))
enriched_docs = (documents_pcoll
| 'join' >> beam.Map(lambda doc, query: enrich_doc(doc, query[doc['key']]),
query=AsDict(additional_data_pcoll)))
Unfortunately, this has one shortcoming, and that's the fact that Python does not currently support arbitrarily large side inputs (it currently loads all of the K-V into a single Python dictionary). If your side-input data is large, then you'll want to avoid this option.
Note This will change in the future, but we can't be sure ATM.
Further Improved
Another way of joining two datasets is to use CoGroupByKey. The loading of documents, and of K-V additional data should not change, but when joining, you'd do something like so:
# Turn the documents into key-value tuples as well[
documents_kv_pcoll = (documents_pcoll
| 'format docs' >> beam.Map(lambda doc: (doc['key'], doc)))
enriched_docs = ({'docs': documents_kv_pcoll, 'additional_data': additional_data_pcoll}
| beam.CoGroupByKey()
| 'enrich' >> beam.Map(lambda x: enrich_doc(x['docs'][0], x['additional_data'][0]))
CoGroupByKey will allow you to use arbitrarily large collections on either side.
Answering your questions
You can see an example of using BigQuery as a side input in the cookbook. As you can see there, the data comes parsed (I believe that it comes in their original data types, but it may come in string/unicode). Check the docs (or feel free to ask) if you need to know more.
Currently, Python streaming is in alpha, and it does not support side inputs; but it does support shuffle features such as CoGroupByKey. Your pipeline using CoGroupByKey should work well in streaming.
A reason to prefer Java over Python is that all these features work in Java (unlimited-size side inputs, streaming side inputs). But it seems that for your use case, Python may have all you need.
Note: The code snippets are approximate, but you should be able to debug them using the DirectRunner.
Feel free to ask for clarification, or to ask about other aspects if you feel like it'd help.
I'm reaching you hoping to find answers about Pentaho data integrator limitation.
I'm currentlty working on a 1 to 1 data source integration and would like to make it n to 1-n. This requires dynamic jobs creation and would like to know if any of came across such issue. My 1 to 1 is working perfectly, it integration form differents data source types (CSV, databases "Mysql, Oracle ...) to same date destination and need to make it n to 1-n.
There is a Metadata Injection Step just for that.
A use case similar to yours is described by Diethard here.
Because it seams that you have a lot of different source format, it may be a good investment to read the use case of Jens, the author of the step, here, which (apart for the automation) is precisely your case.
AFAIK in Pentaho DI, it is not possible to create dynamic transformations for any random data sources. PDI looks for the input columns to be available in the input stream before it loads the data to the target database. For example, if you are using 1 data source (in MySQL) and loading the same to the csv output, the csv output step is expecting the presence of input columns in the data source step (Table input). If you are trying to load any n random data sources you need to define input columns/fields for each of them individually.
Alternatively there are few things which you can explore:
1. Fast Dump in Text File Output step:
There is an option to fast data dump the data set in Text file output step. Here you don't need to define any output column. The input fields will be automatically dumped without formatting as it is. You can use this to map all of the input sources to a csv format and then load it to their targets.
2. Extending Java and Kettle together to build a solution:
PDI allows you to create custom JAVA codes on top of kettle. You can check this blog for more. You can use this idea to create custom code to pass n data sources fields to the kettle as a parameter and execute them. {note: i haven't tried this step, just thinking out loud here}
Hope this helps :)
It this Mapbox blog post, Lauren Budorick shares how they got working a routing engine with OSRM that uses elevation data in order to give cyclists better routes... AMAZING!
I also want to explore the potential of OSRM's routing when plugging in external (user-generated) data, but I'm still having a hard time grasping how OSRM's profiles work. I think I get the main idea, that every way (or node?) is piped into a few functions that, all toghether, scores how good that path is.
But that's it, there are plenty of missing parts in my head, like what do each of the functions Lauren uses in her profile do. If anyone could point me to some more detailed information on how all of this works, you'd make my next week much, much easier :)
Also, in Lauren's post, inside source_function she loads a ./srtm_bayarea.asc file. What does that .asc file looks like? How would one generate a file like that one from, let's say, data stored in a pgsql database? Can we use some other format, like GeoJSON?
Then, when in segment_function she uses things like source.lon and target.lat, are those refered to the raw data stored in the asc file? Or is that file processed into some standard that maps everything to comply it?
As you can see, I'm a complete newbie on routing and maybe GIS in general, but I'd love to learn more about this standards and tools that circle around the OSRM ecosystem. Can you share some tips with me?
I think I get the main idea, that every way (or node?) is piped into a few functions that, all toghether, scores how good that path is.
Right, every way and every node are scored as they are read from an OSM dump to determine passability of a node and speed of a way (used as the scoring heuristic).
A basic description of the data format can be found here. As it reads, data immediately available in ArcInfo ASCII grids includes SRTM data. Currently plaintext ASCII grids are the only supported format. There are several great Python tools for GIS developers that may help in converting other data types to ASCII grids - check out rasterio, for example. Here's an example of a really simple python script to convert NED IMGs to ASCII grids:
import sys
import rasterio as rio
import numpy as np
args = sys.argv[1:]
with rio.drivers():
with rio.open(args[0]) as src:
elev = src.read()[0]
profile = src.profile
def shortify(x):
if x == profile['nodata']:
return -9999
elif x == np.finfo(x).tiny:
return 0
else:
return int(round(x))
out_elev = [map(shortify, row) for row in elev]
with open(args[0] + '.asc', 'a') as dst:
np.savetxt(dst, np.array(out_elev),fmt="%s",delimiter=" ")
source.lon and target.lat e.g: source and target are nodes provided as arguments by the extraction process. Their coordinates are used to look up data at each location during extraction.
Make sure to read thoroughly through the relevant wiki page (already linked).
Feel free alternately to open a Github issue in
https://github.com/Project-OSRM/osrm-backend/issues with OSRM
questions.
I'm trying to do the following in hadoop map/reduce( written in java, linux kernel OS)
Text files 'rules-1' and 'rules-2' (total 3GB in size) contains some rules, each rule are separated by endline character, so the files can be read using readLine() function.
These files 'rules-1' and 'rules-2' needs to be imported as a whole from hdfs in every map function in my cluster i.e. these file are not splittable across different map function.
Input to the mapper's map function is a text file called 'record' (each line is terminated by endline character), so from the 'record' file we get the (key, value) pair. The file is splittable and can be given as input to different map function used in the whole map/reduce process.
What needs to be done is compare each value(i.e. lines from record file) with the rules inside 'rules-1' and 'rules-2'
Problem is, if I pull out each line of rules-1 and rules-2 files to a static arraylist only once, so that each mapper can share the same arraylint and try to compare elements in the arraylist with the each input value from the record file, I get a memory overflow error, since 3GB cannot be stored at a time in the arraylist.
Alternatively, if I import only few lines from the rules-1 and rules-2 files at a time and compare them to each value, map/reduce is taking a lot time to finish its job.
Could you guys provide me any other alternative ideas how can this be done without the memory overflow error? Will it help if I put those file-1 and file-2 inside a hdfs supporting database or something? I'm going out of ideas actually.Would really appreciate if some of you guys could provide me your valuable suggestions.
Iif you input files are small - you can load them into static variables and use rules as an input.
If above is not a case I can suggest the following ways:
a) To give rule-1 and rule-2 high replication factor close to the number of nodes you have. Then you can read from HDFS rule=1 and rule-2 for each record in the input relatively efficient - because it will be sequential read from the local datanode.
b) If you can consider some hash function which, when applied to the rule and to the input string will predict without false negatives that they can match - then you can emit this hash for rules, input record and resolve all possible matches in the reducer. It will be very similar to the way how a join is done using MR
c) I would consider some other optimization techniques like building search trees, or sorting since otherwise the problem looks computationally expensive and will took forever...
On this page find Real-World Cluster Configurations
it will cover file size configuration
You could use the param "mapred.child.java.opts" in conf/mapred-site.xml to increase the memory for your mappers. You might not be able to run as many map slots per server but with more servers in your cluster you could still parallelize your job.
Read the content text file from the MapReduce function and read the keyword text file from the mapper function (for reading your HDFS) and split using StringTokenizer value.toString reading from MapReduce and in your mapper function write HDFS read text file code it will read line-by-line so use two while loops here you compare. Whenever you want data send it to reducer.
Split the 3gb text file into several text files and apply that all text files as usual MapReduce your previous program.
For splitting text file I written Java program and you decide how many lines you want write in each text file.