Errors Installing singularity inside dockerfile - docker

Question

I am trying to run a nextflow pipeline which uses an older version of nextflow (21.04.3) and java version 8. Since I have to use this pipeline on a remote server, therefore I can only use singularity.
As this nextflow pipeline also uses singularity pull calls therefore I need the singularity installed inside the docker image as well. Then, I can convert this image docker image to a singularity image and then I can move it to the remote server.
I am trying to install singularity inside dockerfile but I am getting errors,
This is the dockerfile that I am using,
FROM python:3.8.9-slim
LABEL authors="phil.ewels#scilifelab.se,erik.danielsson#scilifelab.se" \
description="Docker image containing requirements for the nfcore tools"
# Do not pick up python packages from $HOME
ENV PYTHONNUSERSITE=1
# Update pip to latest version
RUN python -m pip install --upgrade pip
# Install dependencies
COPY requirements.txt requirements.txt
RUN python -m pip install -r requirements.txt
# Install Nextflow dependencies
RUN apt-get update \
&& apt-get upgrade -y \
&& apt-get install -y git \
&& apt-get install -y wget
# Create man dir required for Java installation
# and install Java
RUN mkdir -p /usr/share/man/man1 \
&& apt-get install -y openjdk-11-jre \
&& apt-get clean -y && rm -rf /var/lib/apt/lists/*
# Install Singularity
RUN wget -O- http://neuro.debian.net/lists/xenial.us-ca.full | tee /etc/apt/sources.list.d/neurodebian.sources.list && \ apt-key adv --recv-keys --keyserver hkp://pool.sks-keyservers.net:80 0xA5D32F012649A5A9 && \ apt-get update
RUN apt-get install -y singularity-container
# Setup ARG for NXF_VER ENV
ARG NXF_VER=""
ENV NXF_VER ${NXF_VER}
# Install Nextflow
RUN wget https://github.com/nextflow- io/nextflow/releases/download/v21.04.3/nextflow | bash \
&& mv nextflow /usr/local/bin \
&& chmod a+rx /usr/local/bin/nextflow
# Add the nf-core source files to the image
COPY . /usr/src/nf_core
WORKDIR /usr/src/nf_core
# Install nf-core
RUN python -m pip install .
# Set up entrypoint and cmd for easy docker usage
CMD [ "." ]
These are the errors I am getting
Step 9/17 : RUN wget -O- http://neuro.debian.net/lists/xenial.us-ca.full | tee
/etc/apt/sources.list.d/neurodebian.sources.list && \ apt-key adv --recv-keys --
keyserver hkp://pool.sks-keyservers.net:80 0xA5D32F012649A5A9 && \ apt-get update
---> Running in afc3dcbbd1ee
--2022-03-17 17:40:19-- http://neuro.debian.net/lists/xenial.us-ca.full
Resolving neuro.debian.net (neuro.debian.net)... 129.170.233.11
Connecting to neuro.debian.net (neuro.debian.net)|129.170.233.11|:80... connected.
HTTP request sent, awaiting response... 200 OK
Length: 262
Saving to: ‘STDOUT’
0K 100% 18.4M=0s
deb http://neurodeb.pirsquared.org data main contrib non-free
#deb-src http://neurodeb.pirsquared.org data main contrib non-free
deb http://neurodeb.pirsquared.org xenial main contrib non-free
#deb-src http://neurodeb.pirsquared.org xenial main contrib non-free
2022-03-17 17:40:19 (18.4 MB/s) - written to stdout [262/262]
/bin/sh: 1: apt-key: not found
The command '/bin/sh -c wget -O- http://neuro.debian.net/lists/xenial.us-ca.full | tee /etc/apt/sources.list.d/neurodebian.sources.list && \ apt-key adv --recv-keys --keyserver hkp://pool.sks-keyservers.net:80 0xA5D32F012649A5A9 && \ apt-get update'
returned a non-zero code: 127
I there a way to install singularity using a dockerfile ?
Thanks

Answer 1

I made some changes in the dockerfile based on the method to install singularity in linux given here.
The complete dockerfile with which I was able to run successfully nextflow, java and singularity within singularity is given below,
FROM python:3.8.9-slim
LABEL
authors="phil.ewels#scilifelab.se,erik.danielsson#scilifelab.se" \
description="Docker image containing requirements for the nfcore tools"
# Do not pick up python packages from $HOME
ENV PYTHONNUSERSITE=1
# Update pip to latest version
RUN python -m pip install --upgrade pip
# Install dependencies
COPY requirements.txt requirements.txt
RUN python -m pip install -r requirements.txt
# Install Nextflow dependencies
RUN apt-get update \
&& apt-get upgrade -y \
&& apt-get install -y git \
&& apt-get install -y wget
# Create man dir required for Java installation
# and install Java
RUN mkdir -p /usr/share/man/man1 \
&& apt-get install -y openjdk-11-jre \
&& apt-get clean -y && rm -rf /var/lib/apt/lists/*
# Install Singularity
RUN apt-get update && apt-get install -y \
build-essential \
libssl-dev \
uuid-dev \
libgpgme11-dev \
squashfs-tools \
libseccomp-dev \
wget \
pkg-config \
procps
# Download Go source version 1.16.3, install them and modify the PATH
ENV VERSION=1.16.3
ENV OS=linux
ENV ARCH=amd64
RUN wget https://dl.google.com/go/go$VERSION.$OS-$ARCH.tar.gz && \
tar -C /usr/local -xzvf go$VERSION.$OS-$ARCH.tar.gz && \
rm go$VERSION.$OS-$ARCH.tar.gz && \
echo 'export PATH=$PATH:/usr/local/go/bin' | tee -a /etc/profile
# Download Singularity from version 3.7.3 (security version)
ENV VERSION=3.7.3
RUN wget https://github.com/sylabs/singularity/releases/download/v${VERSION}/singularity-${VERSION}.tar.gz && \
tar -xzf singularity-${VERSION}.tar.gz
# Compile Singularity sources and install it
RUN export PATH=$PATH:/usr/local/go/bin && \
cd singularity && \
./mconfig --without-suid && \
make -C ./builddir && \
make -C ./builddir install
# Setup ARG for NXF_VER ENV
ARG NXF_VER=""
ENV NXF_VER ${NXF_VER}
# Install Nextflow
RUN wget https://github.com/nextflow-io/nextflow/releases/download/v21.04.3/nextflow | bash \
&& mv nextflow /usr/local/bin \
&& chmod a+rx /usr/local/bin/nextflow
# Add the nf-core source files to the image
COPY . /usr/src/nf_core
WORKDIR /usr/src/nf_core
# Install nf-core
RUN python -m pip install .
# Set up entrypoint and cmd for easy docker usage
CMD [ "." ]
The file named requirements.txt used in the above dockerfile is given below,
click
GitPython
jinja2
jsonschema
packaging
prompt_toolkit>=3.0.3
pyyaml
pytest-workflow
questionary>=1.8.0
requests_cache
requests
rich>=10.0.0
tabulate