Image standarization technique methods - opencv

I want to know what are the types of image standardizations techniques available. For example, I'm working on hemoglobin concentration detection using photographs in which the patient pull lower conjunctiva downwards with one hand while holding a color calibration card in the other hand. All images are standardized to enable comparison using a previously established method. First, each image was split into its component 8-bit red, green and blue channels. Each channel’s brightness was adjusted by multiplying its brightness by 200/MB where MB is the mean brightness of the color calibration card’s white square. At this point, the channels were duplicated, with one set merged to produce a 24-bit white-balanced image.
This image standardization technique is not perfect and many times give wrong results. Is there any better image standardization technique available. Any idea or right direction will be helpful.
Edit
As i said above I'm working on the hemoglobin detection from a digital photograph. To reduce the effect of ambient lightning a image standardization technique is to be implemented. Current image standardization technique does not reduce the effect of ambient light to a great extent. The color calibration card in the photograph above could be used for the standardization process. I have heard of "white balance algorithm in mars probe" was used to overcome the same problem but was unable to find a proper source that could give enough information to implement it in OpenCV. Another approach or appropriate reference is helpful.

Related

OpenCV Matching Exposure across images

I was wondering if its possible to match the exposure across a set of images.
For example, lets say you have 5 images that were taken at different angles. Images 1-3,5 are taken with the same exposure whilst the 4th image have a slightly darker exposure. When I then try to combine these into a cylindrical panorama using (seamFinder with: gc_color, surf detection, MULTI_BAND blending,Wave correction, etc.) the result turns out with a big shadow in the middle due to the darkness from image 4.
I've also tried using exposureCompensator without luck.
Since I'm taking the pictures in iOS, I maybe could increase exposure manually when needed? But this doesn't seem optimal..
Have anyone else dealt with this problem?
This method is probably overkill (and not just a little) but the current state-of-the-art method for ensuring color consistency between different images is presented in this article from HaCohen et al.
Their algorithm can correct a wide range of errors in image sets. I have implemented and tested it on datasets with large errors and it performs very well.
But, once again, I suppose this is way overkill for panorama stitching.
Sunreef has provided a very good paper, but it does seem overkill because of the complexity of a possible implementation.
What you want to do is to equalize the exposure not on the entire images, but on the overlapping zones. If the histograms of the overlapped zones match, it is a good indicator that the images have similar brightness and exposure conditions. Since you are doing more than 1 stitch, you may require a global equalization in order to make all the images look similar, and then only equalize them using either a weighted equalization on the overlapped region or a quadratic optimiser (which is again overkill if you are not a professional photographer). OpenCV has a simple implmentation of a simple equalization compensation algorithm.
The detail::ExposureCompensator class of OpenCV (sample implementation of such a stitiching is here) would be ideal for you to use.
Just create a compensator (try the 2 different types of compensation: GAIN and GAIN_BLOCKS)
Feed the images into the compensator, based on where their top-left cornes lie (in the stitched image) along with a mask (which can be either completely white or white only in the overlapped region).
Apply compensation on each individual image and iteratively check the results.
I don't know any way to do this in iOS, just OpenCV.

Water Edge Detection

Is there a robust way to detect the water line, like the edge of a river in this image, in OpenCV?
(source: pequannockriver.org)
This task is challenging because a combination of techniques must be used. Furthermore, for each technique, the numerical parameters may only work correctly for a very narrow range. This means either a human expert must tune them by trial-and-error for each image, or that the technique must be executed many times with many different parameters, in order for the correct result to be selected.
The following outline is highly-specific to this sample image. It might not work with any other images.
One bit of advice: As usual, any multi-step image analysis should always begin with the most reliable step, and then proceed down to the less reliable steps. Whenever possible, the less reliable step should make use of the result of more-reliable steps to augment its own accuracy.
Detection of sky
Convert image to HSV colorspace, and find the cyan located at the upper-half of the image.
Keep this HSV image, becuase it could be handy for the next few steps as well.
Detection of shrubs
Run Canny edge detection on the grayscale version of image, with suitably chosen sigma and thresholds. This will pick up the branches on the shrubs, which would look like a bunch of noise. Meanwhile, the water surface would be relatively smooth.
Grayscale is used in this technique in order to reduce the influence of reflections on the water surface (the green and yellow reflections from the shrubs). There might be other colorspaces (or preprocessing techniques) more capable of removing that reflection.
Detection of water ripples from a lower elevation angle viewpoint
Firstly, mark off any image parts that are already classified as shrubs or sky. Since shrub detection would be more reliable than water detection, shrub detection's result should be used to inform the less-reliable water detection.
Observation
Because of the low elevation angle viewpoint, the water ripples appear horizontally elongated. In fact, every image feature appears stretched horizontally. This is called Anisotropy. We could make use of this tendency to detect them.
Note: I am not experienced in anisotropy detection. Perhaps you can get better ideas from other people.
Idea 1:
Use maximally-stable extremal regions (MSER) as a blob detector.
The Wikipedia introduction appears intimidating, but it is really related to connected-component algorithms. A naive implementation can be done similar to Dijkstra's algorithm.
Idea 2:
Notice that the image features are horizontally stretched, a simpler approach is to just sum up the absolute values of horizontal gradients and compare that to the sum of absolute values of vertical gradients.

A suitable workflow to detect and classify blurs in images? [duplicate]

I had asked this on photo stackexchange but thought it might be relevant here as well, since I want to implement this programatically in my implementation.
I am trying to implement a blur detection algorithm for my imaging pipeline. The blur that I want to detect is both -
1) Camera Shake: Pictures captured using hand which moves/shakes when shutter speed is less.
2) Lens focussing errors - (Depth of Field) issues, like focussing on a incorrect object causing some blur.
3) Motion blur: Fast moving objects in the scene, captured using a not high enough shutter speed. E.g. A moving car a night might show a trail of its headlight/tail light in the image as a blur.
How can one detect this blur and quantify it in some way to make some decision based on that computed 'blur metric'?
What is the theory behind blur detection?
I am looking of good reading material using which I can implement some algorithm for this in C/Matlab.
thank you.
-AD.
Motion blur and camera shake are kind of the same thing when you think about the cause: relative motion of the camera and the object. You mention slow shutter speed -- it is a culprit in both cases.
Focus misses are subjective as they depend on the intent on the photographer. Without knowing what the photographer wanted to focus on, it's impossible to achieve this. And even if you do know what you wanted to focus on, it still wouldn't be trivial.
With that dose of realism aside, let me reassure you that blur detection is actually a very active research field, and there are already a few metrics that you can try out on your images. Here are some that I've used recently:
Edge width. Basically, perform edge detection on your image (using Canny or otherwise) and then measure the width of the edges. Blurry images will have wider edges that are more spread out. Sharper images will have thinner edges. Google for "A no-reference perceptual blur metric" by Marziliano -- it's a famous paper that describes this approach well enough for a full implementation. If you're dealing with motion blur, then the edges will be blurred (wide) in the direction of the motion.
Presence of fine detail. Have a look at my answer to this question (the edited part).
Frequency domain approaches. Taking the histogram of the DCT coefficients of the image (assuming you're working with JPEG) would give you an idea of how much fine detail the image has. This is how you grab the DCT coefficients from a JPEG file directly. If the count for the non-DC terms is low, it is likely that the image is blurry. This is the simplest way -- there are more sophisticated approaches in the frequency domain.
There are more, but I feel that that should be enough to get you started. If you require further info on either of those points, fire up Google Scholar and look around. In particular, check out the references of Marziliano's paper to get an idea about what has been tried in the past.
There is a great paper called : "analysis of focus measure operators for shape-from-focus" (https://www.researchgate.net/publication/234073157_Analysis_of_focus_measure_operators_in_shape-from-focus) , which does a comparison about 30 different techniques.
Out of all the different techniques, the "Laplacian" based methods seem to have the best performance. Most image processing programs like : MATLAB or OPENCV have already implemented this method . Below is an example using OpenCV : http://www.pyimagesearch.com/2015/09/07/blur-detection-with-opencv/
One important point to note here is that an image can have some blurry areas and some sharp areas. For example, if an image contains portrait photography, the image in the foreground is sharp whereas the background is blurry. In sports photography, the object in focus is sharp and the background usually has motion blur. One way to detect such a spatially varying blur in an image is to run a frequency domain analysis at every location in the image. One of the papers which addresses this topic is "Spatially-Varying Blur Detection Based on Multiscale Fused and Sorted Transform Coefficients of Gradient Magnitudes" (cvpr2017).
the authors look at multi resolution DCT coefficients at every pixel. These DCT coefficients are divided into low, medium, and high frequency bands, out of which only the high frequency coefficients are selected.
The DCT coefficients are then fused together and sorted to form the multiscale-fused and sorted high-frequency transform coefficients
A subset of these coefficients are selected. the number of selected coefficients is a tunable parameter which is application specific.
The selected subset of coefficients are then sent through a max pooling block to retain the highest activation within all the scales. This gives the blur map as the output, which is then sent through a post processing step to refine the map.
This blur map can be used to quantify the sharpness in various regions of the image. In order to get a single global metric to quantify the bluriness of the entire image, the mean of this blur map or the histogram of this blur map can be used
Here are some examples results on how the algorithm performs:
The sharp regions in the image have a high intensity in the blur_map, whereas blurry regions have a low intensity.
The github link to the project is: https://github.com/Utkarsh-Deshmukh/Spatially-Varying-Blur-Detection-python
The python implementation of this algorithm can be found on pypi which can easily be installed as shown below:
pip install blur_detector
A sample code snippet to generate the blur map is as follows:
import blur_detector
import cv2
if __name__ == '__main__':
img = cv2.imread('image_name', 0)
blur_map = blur_detector.detectBlur(img, downsampling_factor=4, num_scales=4, scale_start=2, num_iterations_RF_filter=3)
cv2.imshow('ori_img', img)
cv2.imshow('blur_map', blur_map)
cv2.waitKey(0)
For detecting blurry images, you can tweak the approach and add "Region of Interest estimation".
In this github link: https://github.com/Utkarsh-Deshmukh/Blurry-Image-Detector , I have used local entropy filters to estimate a region of interest. In this ROI, I then use DCT coefficients as feature extractors and train a simple multi-layer perceptron. On testing this approach on 20000 images in the "BSD-B" dataset (http://cg.postech.ac.kr/research/realblur/) I got an average accuracy of 94%
Just to add on the focussing errors, these may be detected by comparing the psf of the captured blurry images (wider) with reference ones (sharper). Deconvolution techniques may help correcting them but leaving artificial errors (shadows, rippling, ...). A light field camera can help refocusing to any depth planes since it captures the angular information besides the traditional spatial ones of the scene.

why we should use gray scale for image processing

I think this can be a stupid question but after read a lot and search a lot about image processing every example I see about image processing uses gray scale to work
I understood that gray scale images use just one channel of color, that normally is necessary just 8 bit to be represented, etc... but, why use gray scale when we have a color image? What are the advantages of a gray scale? I could imagine that is because we have less bits to treat but even today with faster computers this is necessary?
I am not sure if I was clear about my doubt, I hope someone can answer me
thank you very much
As explained by John Zhang:
luminance is by far more important in distinguishing visual features
John also gives an excellent suggestion to illustrate this property: take a given image and separate the luminance plane from the chrominance planes.
To do so you can use ImageMagick separate operator that extracts the current contents of each channel as a gray-scale image:
convert myimage.gif -colorspace YCbCr -separate sep_YCbCr_%d.gif
Here's what it gives on a sample image (top-left: original color image, top-right: luminance plane, bottom row: chrominance planes):
To elaborate a bit on deltheil's answer:
Signal to noise. For many applications of image processing, color information doesn't help us identify important edges or other features. There are exceptions. If there is an edge (a step change in pixel value) in hue that is hard to detect in a grayscale image, or if we need to identify objects of known hue (orange fruit in front of green leaves), then color information could be useful. If we don't need color, then we can consider it noise. At first it's a bit counterintuitive to "think" in grayscale, but you get used to it.
Complexity of the code. If you want to find edges based on luminance AND chrominance, you've got more work ahead of you. That additional work (and additional debugging, additional pain in supporting the software, etc.) is hard to justify if the additional color information isn't helpful for applications of interest.
For learning image processing, it's better to understand grayscale processing first and understand how it applies to multichannel processing rather than starting with full color imaging and missing all the important insights that can (and should) be learned from single channel processing.
Difficulty of visualization. In grayscale images, the watershed algorithm is fairly easy to conceptualize because we can think of the two spatial dimensions and one brightness dimension as a 3D image with hills, valleys, catchment basins, ridges, etc. "Peak brightness" is just a mountain peak in our 3D visualization of the grayscale image. There are a number of algorithms for which an intuitive "physical" interpretation helps us think through a problem. In RGB, HSI, Lab, and other color spaces this sort of visualization is much harder since there are additional dimensions that the standard human brain can't visualize easily. Sure, we can think of "peak redness," but what does that mountain peak look like in an (x,y,h,s,i) space? Ouch. One workaround is to think of each color variable as an intensity image, but that leads us right back to grayscale image processing.
Color is complex. Humans perceive color and identify color with deceptive ease. If you get into the business of attempting to distinguish colors from one another, then you'll either want to (a) follow tradition and control the lighting, camera color calibration, and other factors to ensure the best results, or (b) settle down for a career-long journey into a topic that gets deeper the more you look at it, or (c) wish you could be back working with grayscale because at least then the problems seem solvable.
Speed. With modern computers, and with parallel programming, it's possible to perform simple pixel-by-pixel processing of a megapixel image in milliseconds. Facial recognition, OCR, content-aware resizing, mean shift segmentation, and other tasks can take much longer than that. Whatever processing time is required to manipulate the image or squeeze some useful data from it, most customers/users want it to go faster. If we make the hand-wavy assumption that processing a three-channel color image takes three times as long as processing a grayscale image--or maybe four times as long, since we may create a separate luminance channel--then that's not a big deal if we're processing video images on the fly and each frame can be processed in less than 1/30th or 1/25th of a second. But if we're analyzing thousands of images from a database, it's great if we can save ourselves processing time by resizing images, analyzing only portions of images, and/or eliminating color channels we don't need. Cutting processing time by a factor of three to four can mean the difference between running an 8-hour overnight test that ends before you get back to work, and having your computer's processors pegged for 24 hours straight.
Of all these, I'll emphasize the first two: make the image simpler, and reduce the amount of code you have to write.
I disagree with the implication that gray scale images are always better than color images; it depends on the technique and the overall goal of the processing. For example, if you wanted to count the bananas in an image of a fruit bowl image, then it's much easier to segment when you have a colored image!
Many images have to be in grayscale because of the measuring device used to obtain them. Think of an electron microscope. It's measuring the strength of an electron beam at various space points. An AFM is measuring the amount of resonance vibrations at various points topologically on a sample. In both cases, these tools are returning a singular value- an intensity, so they implicitly are creating a gray-scale image.
For image processing techniques based on brightness, they often can be applied sufficiently to the overall brightness (grayscale); however, there are many many instances where having a colored image is an advantage.
Binary might be too simple and it could not represent the picture character.
Color might be too much and affect the processing speed.
Thus, grayscale is chosen, which is in the mid of the two ends.
First of starting image processing whether on gray scale or color images, it is better to focus on the applications which we are applying. Unless and otherwise, if we choose one of them randomly, it will create accuracy problem in our result. For example, if I want to process image of waste bin, I prefer to choose gray scale rather than color. Because in the bin image I want only to detect the shape of bin image using optimized edge detection. I could not bother about the color of image but I want to see rectangular shape of the bin image correctly.

Detection of Blur in Images/Video sequences

I had asked this on photo stackexchange but thought it might be relevant here as well, since I want to implement this programatically in my implementation.
I am trying to implement a blur detection algorithm for my imaging pipeline. The blur that I want to detect is both -
1) Camera Shake: Pictures captured using hand which moves/shakes when shutter speed is less.
2) Lens focussing errors - (Depth of Field) issues, like focussing on a incorrect object causing some blur.
3) Motion blur: Fast moving objects in the scene, captured using a not high enough shutter speed. E.g. A moving car a night might show a trail of its headlight/tail light in the image as a blur.
How can one detect this blur and quantify it in some way to make some decision based on that computed 'blur metric'?
What is the theory behind blur detection?
I am looking of good reading material using which I can implement some algorithm for this in C/Matlab.
thank you.
-AD.
Motion blur and camera shake are kind of the same thing when you think about the cause: relative motion of the camera and the object. You mention slow shutter speed -- it is a culprit in both cases.
Focus misses are subjective as they depend on the intent on the photographer. Without knowing what the photographer wanted to focus on, it's impossible to achieve this. And even if you do know what you wanted to focus on, it still wouldn't be trivial.
With that dose of realism aside, let me reassure you that blur detection is actually a very active research field, and there are already a few metrics that you can try out on your images. Here are some that I've used recently:
Edge width. Basically, perform edge detection on your image (using Canny or otherwise) and then measure the width of the edges. Blurry images will have wider edges that are more spread out. Sharper images will have thinner edges. Google for "A no-reference perceptual blur metric" by Marziliano -- it's a famous paper that describes this approach well enough for a full implementation. If you're dealing with motion blur, then the edges will be blurred (wide) in the direction of the motion.
Presence of fine detail. Have a look at my answer to this question (the edited part).
Frequency domain approaches. Taking the histogram of the DCT coefficients of the image (assuming you're working with JPEG) would give you an idea of how much fine detail the image has. This is how you grab the DCT coefficients from a JPEG file directly. If the count for the non-DC terms is low, it is likely that the image is blurry. This is the simplest way -- there are more sophisticated approaches in the frequency domain.
There are more, but I feel that that should be enough to get you started. If you require further info on either of those points, fire up Google Scholar and look around. In particular, check out the references of Marziliano's paper to get an idea about what has been tried in the past.
There is a great paper called : "analysis of focus measure operators for shape-from-focus" (https://www.researchgate.net/publication/234073157_Analysis_of_focus_measure_operators_in_shape-from-focus) , which does a comparison about 30 different techniques.
Out of all the different techniques, the "Laplacian" based methods seem to have the best performance. Most image processing programs like : MATLAB or OPENCV have already implemented this method . Below is an example using OpenCV : http://www.pyimagesearch.com/2015/09/07/blur-detection-with-opencv/
One important point to note here is that an image can have some blurry areas and some sharp areas. For example, if an image contains portrait photography, the image in the foreground is sharp whereas the background is blurry. In sports photography, the object in focus is sharp and the background usually has motion blur. One way to detect such a spatially varying blur in an image is to run a frequency domain analysis at every location in the image. One of the papers which addresses this topic is "Spatially-Varying Blur Detection Based on Multiscale Fused and Sorted Transform Coefficients of Gradient Magnitudes" (cvpr2017).
the authors look at multi resolution DCT coefficients at every pixel. These DCT coefficients are divided into low, medium, and high frequency bands, out of which only the high frequency coefficients are selected.
The DCT coefficients are then fused together and sorted to form the multiscale-fused and sorted high-frequency transform coefficients
A subset of these coefficients are selected. the number of selected coefficients is a tunable parameter which is application specific.
The selected subset of coefficients are then sent through a max pooling block to retain the highest activation within all the scales. This gives the blur map as the output, which is then sent through a post processing step to refine the map.
This blur map can be used to quantify the sharpness in various regions of the image. In order to get a single global metric to quantify the bluriness of the entire image, the mean of this blur map or the histogram of this blur map can be used
Here are some examples results on how the algorithm performs:
The sharp regions in the image have a high intensity in the blur_map, whereas blurry regions have a low intensity.
The github link to the project is: https://github.com/Utkarsh-Deshmukh/Spatially-Varying-Blur-Detection-python
The python implementation of this algorithm can be found on pypi which can easily be installed as shown below:
pip install blur_detector
A sample code snippet to generate the blur map is as follows:
import blur_detector
import cv2
if __name__ == '__main__':
img = cv2.imread('image_name', 0)
blur_map = blur_detector.detectBlur(img, downsampling_factor=4, num_scales=4, scale_start=2, num_iterations_RF_filter=3)
cv2.imshow('ori_img', img)
cv2.imshow('blur_map', blur_map)
cv2.waitKey(0)
For detecting blurry images, you can tweak the approach and add "Region of Interest estimation".
In this github link: https://github.com/Utkarsh-Deshmukh/Blurry-Image-Detector , I have used local entropy filters to estimate a region of interest. In this ROI, I then use DCT coefficients as feature extractors and train a simple multi-layer perceptron. On testing this approach on 20000 images in the "BSD-B" dataset (http://cg.postech.ac.kr/research/realblur/) I got an average accuracy of 94%
Just to add on the focussing errors, these may be detected by comparing the psf of the captured blurry images (wider) with reference ones (sharper). Deconvolution techniques may help correcting them but leaving artificial errors (shadows, rippling, ...). A light field camera can help refocusing to any depth planes since it captures the angular information besides the traditional spatial ones of the scene.

Resources